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Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica acquires resistance to salt stress in transgenic tobacco plants. Plant Science (Shannon) 146, 81-88.
| Sugino M., Hibino, T., Tanaka, Y., Nii, N., Takabe, Tetsuko, Takabe, T. (1999).
| To test the role of the heat shock protein DnaK/Hsp70 in salt tolerance, transgenic plants of Nicotina tabacum cv Petit Havana SR1 were made with DnaK1 from a halotolerant cyanobacterium Aphanothece halophytica (A. halophytica) overexpressed in the cytosol. The growth rate and photosynthetic activities of the transgenic and control tobacco plants were similar under non-stressed conditions. The CO2 assimilation rate of the control plants decreased with increasing concentration of NaCl. After 3 days of treatment with 0.6 M NaCl, the CO2 fixation rate decreased to 40% of that in the non-stressed plants whereas its activity in the transgenic plants was about 85% of that in the non-stressed plants. Similar results were observed for the stomatal transpiration. The sodium contents in leaves of the control plants were significantly increased by salt stress whereas those in the transgenic plants remained at levels similar to those in the non-stressed plants. Total protein contents and ribulose 1.5-bis phosphate carboxygenase and oxygenase (RuBisCO) levels were decreased by salt stress in both the transgenic and control plants but the decrease was slight in the transgenic tobacco. All these data clearly indicate that the expression of DnaK1 from a halotolerant cyanobacterium A. halophytica improved the salt tolerance of the tobacco plant.
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