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Cyt1Aa protein of Bacillus thuringiensis is toxic to the cottonwood leaf beetle, Chrysomela scripta, and suppresses high levels of resistance to Cry3Aa. Applied and Environmental Microbiology 64, 4368-4371.
| Federici B.A., Bauer, Leah S. (1998).
| The insecticidal activity of Bacillus thuringiensis is due primarily to Cry and Cyt proteins. Cry proteins are typically toxic to lepidopterous, coleopterous, or dipterous insects, whereas the known toxicity of Cyt proteins is limited to dipterans. We report here that a Cyt protein, Cyt1Aa, is also highly toxic to the cottonwood leaf beetle, Chrysomela scripta, with a median lethal concentration of 2.5 ng/mm2 of leaf surface for second-instar larvae. Additionally, we show that Cyt1Aa suppresses resistance to Cry3Aa greater than 5,000-fold in C. scripta, a level only partially overcome by Cry1Ba due to cross-resistance. Studies of the histopathology of C. scripta larvae treated with Cyt1Aa revealed disruption and sloughing of midgut epithelial cells, indicating that its mechanism of action against C. scripta is similar to that observed in mosquito and blackfly larvae. These novel properties suggest that Cyt proteins may have an even broader spectrum of activity against insects and, owing to their different mechanism of action in comparison to Cry proteins, might be useful in managing resistance to Cry3 and possibly other Cry toxins used in microbial insecticides and transgenic plants.
Cyt1As, a cyt protein with insecticidal activity of Bacillus thuringiensis is shown to be highly toxic to the cottonwood leaf beetle, Chrysomela scripta. Cyt1Aa additionally suppresses resistance to Cry3Aa greater than 5000-fold in C.scripta, a level only partially overcome by Cry1Ba due to cross-resistance. Cyt proteins might be useful in managing resistance to Cry toxins used in microbial insecticides and transgenic plants.
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