|
> Wissenschaftliche Publikationen <
|
Regeneration and Agrobacterium-mediated transformation of Forsythia X intermedia "Spring Glory". Plant Cell Reports 16, 114-117.
| Rosati C., Cadic, Alain, Renou, Jean-Pierre, Duron, Michel (1996).
| Internode explants of in vitro plants of Forsythia times intermedia "Spring Glory" were transformed with the gus and npt II genes after inoculation with the A. tumefaciens strain EHA 101 harbouring the plasmid pFAJ3000. Shoot organogenesis took place from callused edges of explants. The first transformed buds were detected 4 to 6 weeks after transfer on regeneration medium, containing 25 mg/l kanamycin as selective agent. An average of 1% of explants regenerated transgenic shoots. beta-glucuronidase assays and culture on kanainycin containing medium provided the first indication of integration and expression of introduced genes in transformants. Southern blot and polymerase chain reaction amplification analyses gave molecular confirmation of genetic transformation. Transgenic plants were acclimatized in the greenhouse. Enzymatic assays on several organs of mature plants still showed beta-glucuronidase activity, thus confirming stable integration of T-DNA in the plant genome.
| | Zurück |
|
|
Interviews mit Forschern
Erfahren Sie mehr über einzelne Forschungsprojekte und die Leute, die dahinterstehen. | 
Gentechnik im Klassenzimmer
Aufgaben zum Thema Gentechnik, die in den Schulunterricht integriert werden können. | 
Genomik und Proteomik
Wie man heute die Gene und Proteine eines Organismus untersucht. | 
Forschungseinblicke
Verfolgen Sie die Arbeit an aktuellen Projekten, z.B. am Weizen, Fadenwurm oder in der Krebsforschung. |
|
