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Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase. Theoretical and Applied Genetics 99, 383-390.
| Nishizawa Y., Nishio, Z., Nakazono, K., Soma, M., Nakajima, E., Ugaki, M., Hibi, T. (1999).
| Rice blast is the most devastating plant disease in Japan. Our goal is to create new rice varieties which show enhanced resistance against blast, regardless of the race of blast. By an Agrobacterium-mediated transformation method, we reintroduced a rice class-I chitinase gene, Cht-2 or Cht-3, under the control of the enhanced CaMV 35S promoter and a hygromycin phosphotransferase gene, as a selection marker into the Japonica rice varieties Nipponbare and Koshihikari, which have retained the best popularity over a long period in Japan. In regenerated plants (R0), the Cht-2 product was found to accumulate intracellularly whereas the Cht-3 product was found to be targeted extracellularly. The transgenic rice plants which constitutively expressed either chitinase gene showed significantly higher resistance against the rice blast pathogen Magnaporthe grisea races 007.0 and 333. Both high-level expression of the chitinase and blast-resistance were stably inherited by the next generation in several lines.
Rice blast is the most devastating plant disease in Japan, which results in severe damage, especially where rice is irrigated or receives high amounts of rainfall and high levels of nitrogen fertilizer. Two different rice class-I chitinase genes were introduced into two popular Japonica rice varieties. The transgenic rice plants, which constitutively expressed either chitinase gene, showed significantly higher resistance against two rice blast pathogen Magnaporthe grisea races.
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