|
> Wissenschaftliche Publikationen <
|
Expression of a sulfur-rich maize seed storage protein, delta-zein, in white clover (Trifolium repens) to improve forage quality. Molecular Breeding 4, 435-448.
| Sharma S.B., Hancock, Kerrie R., Ealing, Paul M., White, Derek W. R. (1998).
| A modified gene encoding a sulfur-rich maize seed storage protein, delta-zein, was introduced into white clover plants by Agrobacterium-mediated transformation. Expression of the gene was under the control of the double 35S promoter of cauliflower mosaic virus and the nopaline synthase gene transcription terminator. All of the transgenic plants expressing transgene-specific mRNA also accumulated delta-zein in their leaves. Levels of the HA epitope tagged delta-zein in the first fully expanded young leaves of different transgenic plants varied from 0.06 to 0.3% of total water-soluble protein. Expression of the protein was also detected in petioles, nodes, internodes, roots and seeds of the transgenic plants. N-terminal sequencing of the modified delta-zein from transgenic plants revealed that the protein is processed in white clover leaves as in maize seeds. All the transgenic plants expressing the delta-zein showed monogenic inheritance of the linked nptII gene conferring kanamycin resistance. The epitope tagged 8zein is relatively stable in white clover leaves and in the highest expressing plants, its accumulation increased with increasing leaf age from 0.3 (youngest leaves) to 1.3% (oldest leaves) of total water-soluble protein. These results open up the possibility of using sulfur-rich and rumen-protected delta-zein to improve white clover forage quality.
A modified gene encoding a sulfur-rich maize seed storage protein, delta-zein, was introduced into white clover, producing levels of 0.06 to 0.3% of total water-soluble protein. Its accumulation increase with leaf age from 0.3% to 1.3% of total water-soluble protein in oldest leaves. These levels are still too low to provide the 300-600 mg per day methionine or cysteine to sheep, to obtain a 20% increase in wool production. Levels of delta-zein will be further increase by crossing the two most highly expresing transgenic white clover lines.
| | Zurück |
|
|
Interviews mit Forschern
Erfahren Sie mehr über einzelne Forschungsprojekte und die Leute, die dahinterstehen. | 
Gentechnik im Klassenzimmer
Aufgaben zum Thema Gentechnik, die in den Schulunterricht integriert werden können. | 
Genomik und Proteomik
Wie man heute die Gene und Proteine eines Organismus untersucht. | 
Forschungseinblicke
Verfolgen Sie die Arbeit an aktuellen Projekten, z.B. am Weizen, Fadenwurm oder in der Krebsforschung. |
|
