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Salt tolerance of transgenic rice overexpressing yeast mitochondrial Mn-SOD in chloroplasts. Plant Science (Shannon) 148, 131-138.
| Tanaka Y., Hibino, T., Hayashi, Y., Tanaka, A., Kishitani, S., Yokota, S., Takabe, T. (1999).
| The potential role of superoxide dismutase (SOD) in the protection against salt stress was examined using transgenic rice plants. The coding region of the yeast mitochondrial Mn-SOD gene was fused with the chloroplast targeting signal of glutamine synthetase gene and introduced into rice protoplasts by electroporation. Immunogold labeling experiments revealed that the yeast Mn-SOD was accumulated in the chloroplasts of transgenic rice. The total SOD activity in the control plant was mostly attributed to the activity of cytosolic and chloroplastic Cu/Zn-SOD. Total SOD activity in the transformant was about 1.7-fold that of the control under non-stressed conditions. The photosynthetic electron transport rates of control and transgenic rice were similar under non-stressed conditions. Upon salt stress (100 mM NaCl), the SOD activities decreased in both plants, but the decrease was faster in the control plant. The activities of overexpressed Mn-SOD and cytosolic Cu/Zn-SOD did not change upon salt stress in either the transgenic or control plants, whereas the chloroplastic Cu/Zn-SOD activity in control rice decreased significantly. At high salinity, the ascorbate peroxidase activity of the transformant was about 1.5-fold higher than that in the control. These results suggest that increased levels of ascorbate peroxidase and high levels of chloroplastic SOD in the transformant are important factors for salt resistance in rice.
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